Stereoselective biotransformation of racemic mandelic acid using immobilized laccase and (S)-mandelate dehydrogenase

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Stereoselective biotransformation of racemic mandelic acid using immobilized laccase and (S)-mandelate dehydrogenase

OBJECTIVES (S)-Mandelate dehydrogenase (SMDH) and laccase were immobilized on chitosan. The bi-enzymatic system with immobilized SMDH and immobilized laccase was taken to catalyze the stereoselective transformation of racemic mandelic acid and (R)-mandelic acid was obtained from its racemic mixture. RESULTS Characteristics of the immobilized enzymes were valuated. The optimum pH and temperatu...

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Production of optically pure products can be based on simple unselective synthesis of racemic mixtures combined with a subsequent separation of the enantiomers. However, this approach suffers from a 50% yield limitation which can be overcome by racemization of the undesired enantiomer and recycling. Application of biocatalyst for the racemization steps offers an attractive option for high-yield...

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Magnesium Bis[D(-)-Mandelate] Dihydrate and Other Alkaline Earth, Alkali, and Zinc Salts of Mandelic Acid

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L-mandelate dehydrogenase activity

Flavocytochrome b # from Saccharomyces cereŠisiae is an lactate dehydrogenase which exhibits only barely detectable activity levels towards another 2-hydroxyacid, -mandelate. Using protein engineering methods we have altered the active site of flavocytochrome b # and successfully introduced substantial mandelate dehydrogenase activity into the enzyme. Changes to Ala-198 and Leu-230 have signi...

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Immobilized cells of a recombinant Escherichia coli expressing nitrilase from Pseudomonas putida were used to catalyze the hydrolysis of mandelonitrile (2hydroxy-2-phenylacetonitrile) to (R)-(-)-mandelic acid. The cells had been immobilized by entrapment in an alginate matrix. Conditions for the hydrolysis reaction were optimized in shake flasks and in a packed bed reactor. In shake flasks the ...

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ژورنال

عنوان ژورنال: Bioresources and Bioprocessing

سال: 2017

ISSN: 2197-4365

DOI: 10.1186/s40643-016-0135-3